Amino Acid Limitation Induces the Amino Acid Response (AAR) Pathway in a Time-and Species- Specific Manner

Heather Anderson, Huan Wang, Qian Li, Yuan-Xiang Pan, Hong Chen

Abstract


Amino acids are critical to life and play central roles both as building blocks of proteins and as intermediates in metabolism. The amino acid response (AAR) pathway is activated by lacking of amino acids (i.e. amino acid starvation). We hypothesized that the activation of AAR pathway might occur in a time-dependent manner in hepatoma cells, which might induce restriction of protein production from DNA. Real-time RT-PCR was used to investigate mRNA expressions during this process. Our results showed that expression of Atf3, Chop, and Asns genes were induced significantly by amino acid deprivation in a time- and species- dependent manner.

Full Text:

PDF

Refbacks

  • There are currently no refbacks.


Copyright (c) 2015 Heather Anderson, Huan Wang, Qian Li, Yuan-Xiang Pan, Hong Chen


Undergraduate research journals at the University of Illinois at Urbana-Champaign are supported by the Scholarly Commons and the Office of Undergraduate Research.

To learn more about undergraduate research activities and events on the University of Illinois campus, please visit: Undergraduate Research at Illinois.


 

University of Illinois at Urbana-ChampaignOpen Access